Chemical and Biochemical Properties of Humic Substances ...
The humic substances were extracted from the air-dried samples with 0.1 M KOH (1:20 w/v) at room temperature for 16 h under a N 2 atmosphere and were freed from the suspended material by centrifugation at 7000 × g for 20 min. Humic extracts (50 mL) were transferred into 18000 mol. wt cut-off dialysis tubing (Visking, London) and dialyzed ...